Identification of Dihydrouridine in Escherichia Coli
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چکیده
The N3 imino units of dihydrouridine were identified in samples of I5N-Iabeled Escherichia coli tRNAret, tRNALy., and tRNAPhe by IH_15N two-dimensional NMR. The peaks for dihydrouridine had high field 1 H (9.7-9.8 ppm) and I5N (147.8-149.5 ppm) chemical shifts. Assignments were made by IH_IoN chemical shift correlation based on values obtained in model studies with tri-O-benzoyland tri-O-acetyldihydrouridine. The rates of exchange of the imino protons with water suggest that the D-loop in tRNAret is less stable than the D-loops in tRNALys or tRNAPhe. Closely spaced peaks were observed for the two dihydrouridines in tRNAPhe in a high resolution spectrum.
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تاریخ انتشار 2012