Identification of Dihydrouridine in Escherichia Coli

نویسندگان

  • H. Griffey
  • Ziro Yamaizumi
  • Susumu Nishimura
  • C. Dale
چکیده

The N3 imino units of dihydrouridine were identified in samples of I5N-Iabeled Escherichia coli tRNAret, tRNALy., and tRNAPhe by IH_15N two-dimensional NMR. The peaks for dihydrouridine had high field 1 H (9.7-9.8 ppm) and I5N (147.8-149.5 ppm) chemical shifts. Assignments were made by IH_IoN chemical shift correlation based on values obtained in model studies with tri-O-benzoyland tri-O-acetyldihydrouridine. The rates of exchange of the imino protons with water suggest that the D-loop in tRNAret is less stable than the D-loops in tRNALys or tRNAPhe. Closely spaced peaks were observed for the two dihydrouridines in tRNAPhe in a high resolution spectrum.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

IDENTIFICATION OF 4-THIOURIDINE IN ESCHERICHIA COLI tRNMer AND tRNAiyr

Uridine is uniquely conserved at position B in elongator tRN As and binds to A 14 to form a reversed Hoogsteen base pair which folds the dihydrouridine loop back into the core of the L-shaped molecule. On the basis of IH NMR studies, Hurd and co-workers (Hurd, R. E., Robillard, G. T., and Reid, B. R. (1977) Biochemistry 16,2095-2100) concluded that the interaction between positions Band 14 is a...

متن کامل

Identification of β-Lactamase-Negative-Ampicillin Resistance Strains of Escherichia coli in 150 Isolates from Urinary Tract Infection and Fecal Flora in Kerman

β-lactamases are enzymes which inactivate the β-lactam antibacterial agents and are one of the major causes of resistance against these drugs. Recently there are reports on the isolation of bacteria which does not produce β-lactamase, but are resistant to penicillins. In the present study, β-laclamase production was determined using iodometric method on 150 ampicillin resistance Escherichia col...

متن کامل

Identification of shiga toxin producing Escherichia coli O157:H7 in raw cow milk samples from dairy farms in Mashhad using multiplex PCR assay

In this study 130 bulk tank milk samples which were delivered to the Pegah Pasturisation Factory inMashhad were collected randomly during the summer months. Samples were firstly enriched in modifiedtrypticase soy broth containing novobiocin, followed by plating onto sorbitol MacConkey agar supplementedwith cefixime and potassium tellurite for isolation of Escherichia coli O157:H7. Consequently ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2012